Assay of Pungent Compounds

PID	Structure	Name	IC50	EC50	Assay Organism	Target Name	Assay_References	Note
PID00037		Trimethylamine	-	2100 nM	Mus musculus	Trace amine-associated receptor 5	CHEMBL3784953	Agonist activity at mouse TAAR5 expressed in HEK293 cells assessed as increase in cAMP release after 30 mins by BRET assay relative to control
PID00039		o-Methoxycinnamaldehyde	420000 nM	-	Agaricus bisporus	Tyrosinase	J. Nat. Prod., (2009) 72:6:1205	Inhibition of mushroom tyrosinase after 10 mins by spectrophotometry
PID00039		o-Methoxycinnamaldehyde	13.8 ug.mL-1	-	Homo sapiens	Xanthine dehydrogenase	Bioorg. Med. Chem. Lett., (2012) 22:14:4625	Inhibition of xanthine oxidase- mediated uric acid formation after 5 mins by spectrophotometry
PID00042		Ethylcinnamate	31590 nM	-	Homo sapiens	A-375	Bioorg. Med. Chem. Lett., (2009) 19:21:6078	Cytotoxicity against human A375 cells by MTT assay
PID00042		Ethylcinnamate	41230 nM	-	Homo sapiens	THP-1	Bioorg. Med. Chem. Lett., (2009) 19:21:6078	Cytotoxicity against human THP1 cells by MTT assay
PID00042		Ethylcinnamate	35690 nM	-	Homo sapiens	HL-60	Bioorg. Med. Chem. Lett., (2009) 19:21:6078	Cytotoxicity against human HL60 cells by MTT assay
PID00042		Ethylcinnamate	50350 nM	-	Homo sapiens	A549	Bioorg. Med. Chem. Lett., (2009) 19:21:6078	Cytotoxicity against human A549 cells by MTT assay
PID00042		Ethylcinnamate	95 ug.mL-1	-	Homo sapiens	HUVEC	Eur. J. Med. Chem., (2011) 46:11:5498	Antiinflammatory activity against TNF-alpha-induced ICAM1 protein expression in HUVEC pretreated for 2 hrs before TNFalpha challenge measured after 16 hrs by whole cell ELISA
PID00063		Diallyl Trisulfide	-	4.9 μM	Homo sapiens	Transient receptor potential cation channel subfamily A member 1	Koizumi, K., Iwasaki, Y., Narukawa, M., Iitsuka, Y., Fukao, T., Seki, T., Watanabe, T. Diallyl sulfides in garlic activate both TRPA1 and TRPV1[J]. Biochemical and biophysical research communications, 2009, 382(3): 545-548.	-